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Capsule Staining Technique by Maneval’s Method, Requirement, Procedure

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Capsule Staining Technique by Maneval’s Method. Principle   The principle of capsule staining is based on staining of background with an acidic stain and staining of bacterial cell with a basic stain. A capsule being non-ionic will not stain by either of the two dyes. Thus, capsule staining creates contrast by staining a bacterial cell along with its background and leaving a capsule as a  colorless halo . The other approach of capsule staining is to stain the capsule by leaving a bacterial cell and background colorless. Differential capsule stains are available to highlight the specific structure (like capsule) in a bacterial cell. Requirement 1 % Congo red solution. Maneval’s stain. Capsulated cell culture. Procedure Take a loopful of capsulated cell suspension on a clean grease-free slide. Add a drop of 1 % congo red solution in the suspension and then spread it gently on the slide to form a smear. Allow the suspension to air dry and do not heat fix it. Flood the slid with Maneval’s

ACID FAST STAINING - ZN STAINING PRINCIPLE , PROCEDURE,OBSERVATION

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GRAM'S STAINING - PRINCIPLE , PROCEDURE,OBSERVATION

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